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| Chemical Fingerprinting |
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| In addition to the standard liquid chromatography (HPLC) with UV-VIS or MS detection for quantitative detection of single marker standards, PhytoCeutica has developed a rapid method to identify and characterize a broad range of low molecular weight phytochemicals. This method combines high sensitivity capillary HPLC coupled with high resolution mass spectrometry to provide a high resolution molecular fingerprint that, when coupled to a comprehensive database of phytochemicals, can be used both for accurate phytochemical identification and for the creation of detailed phytochemical fingerprints for QC comparisons. These fingerprints can form the foundation as a signature profile for standardizing the botanical extract that defines the clinical batch for later batch-to-batch comparisons, as a reference to study stability, and as a comprehensive dataset to identify potential adulterated drugs or chemical contaminants. An illustration of the approach is described in Figure 1, using our drug candidate PHY906. |
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| PhytoCeutica’s software package, PhytoViewerCA™, has been written to analyze chemical fingerprint patterns, identify the compounds, define the signature pattern, calculate similarity coefficients, and assign quantitative pattern cut-offs of acceptance or rejection of manufactured batches. |
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| Figure 1: LC/MS
characterization of PHY906. Utilizing high resolution MS
(green), a far more detailed spectrum can be obtained of the phytochemicals
present in PHY906 than with conventional UV/VIS detection (red). This information rich methodology allows the identification
of more than 90 of the observed 125 phytochemical peaks. Note how
single peaks in the UV/VIS trace (red) can be resolved into multiple
phytochemical peaks in the high-resolution mass dimension |
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